4N3G
Crystal structure of eukaryotic translation initiation factor eIF5B (870-1116) from Chaetomium thermophilum, domains III and IV
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-04-08 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.826580 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 98.230, 98.230, 97.420 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.857 - 3.203 |
R-factor | 0.1915 |
Rwork | 0.190 |
R-free | 0.21950 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.611 |
Data scaling software | XSCALE |
Phasing software | PHASER (2.3.0) |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 99.795 | 3.300 | |
High resolution limit [Å] | 3.200 | 50.000 | 3.200 |
Rmerge | 0.034 | 0.574 | |
Number of reflections | 9288 | 800 | |
<I/σ(I)> | 32.12 | 4.31 | |
Completeness [%] | 99.8 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 277 | 100 mM MES, 12% PEG 20000, 10 mM Na-lactate, pH 6.8, VAPOR DIFFUSION, SITTING DROP, temperature 277K |