4N2O
Structure of a novel autonomous cohesin protein from Ruminococcus flavefaciens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-06-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9762 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 116.240, 67.726, 258.907 |
Unit cell angles | 90.00, 93.08, 90.00 |
Refinement procedure
Resolution | 22.770 - 2.442 |
R-factor | 0.2066 |
Rwork | 0.205 |
R-free | 0.22889 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.529 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXS |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 22.770 |
High resolution limit [Å] | 2.440 |
Rmerge | 0.140 |
Number of reflections | 72856 |
<I/σ(I)> | 24 |
Completeness [%] | 97.8 |
Redundancy | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 9 ul drop containing 2 ul of SeMet-RfCohG-CH solution (13mg/ml) and 7 ul reservoir solution (0.1 M Sodium Acetate pH 5 and 6% w/v Polyethylene glycol 4000), VAPOR DIFFUSION, HANGING DROP, temperature 293K |