4N2B
Crystal structure of Protein Arginine Deiminase 2 (10 mM Ca2+)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Detector technology | CCD |
| Collection date | 2003-01-01 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9787 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 202.665, 51.549, 76.217 |
| Unit cell angles | 90.00, 105.76, 90.00 |
Refinement procedure
| Resolution | 40.393 - 1.690 |
| R-factor | 0.1683 |
| Rwork | 0.168 |
| R-free | 0.20020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4n25 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.337 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.393 | |
| High resolution limit [Å] | 1.690 | 1.690 |
| Rmerge | 0.078 | 0.523 |
| Number of reflections | 79078 | |
| <I/σ(I)> | 17.6 | 2.4 |
| Completeness [%] | 99.4 | |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 10-20% MPD, 50 mM MES, pH 5.6, 0.12 M sodium acetate, VAPOR DIFFUSION, SITTING DROP |
