4N25
Crystal structure of Protein Arginine Deiminase 2 (250 uM Ca2+)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Detector technology | CCD |
| Collection date | 2004-01-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 202.276, 51.536, 75.992 |
| Unit cell angles | 90.00, 105.56, 90.00 |
Refinement procedure
| Resolution | 36.603 - 1.931 |
| R-factor | 0.1811 |
| Rwork | 0.179 |
| R-free | 0.22120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4n20 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.221 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.603 | |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.084 | 0.436 |
| Number of reflections | 54984 | |
| <I/σ(I)> | 16.5 | 2 |
| Completeness [%] | 96.6 | |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 10-20% MPD, 50 mM MES, pH 5.6, 0.12 M sodium acetate, VAPOR DIFFUSION, SITTING DROP |
