4N23
Crystal structure of the GP2 Core Domain from the California Academy of Science Virus, monoclinic symmetry
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-12-07 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.9791 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 80.866, 46.718, 109.474 |
Unit cell angles | 90.00, 104.28, 90.00 |
Refinement procedure
Resolution | 39.210 - 2.000 |
R-factor | 0.1788 |
Rwork | 0.176 |
R-free | 0.22750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4n21 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.123 |
Data reduction software | HKL-3000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.5.1) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.030 |
High resolution limit [Å] | 2.000 | 5.430 | 2.000 |
Rmerge | 0.072 | 0.046 | 0.491 |
Number of reflections | 27014 | ||
<I/σ(I)> | 11.1 | ||
Completeness [%] | 99.8 | 98.1 | 99.9 |
Redundancy | 3.7 | 3.6 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | MCSG4-D10 - 0.2 M lithium sulfate, 0.1 M MES-NaOH, pH 6, 35% MPD, vapor diffusion, hanging drop, temperature 298K, VAPOR DIFFUSION, HANGING DROP |