4N1I

Crystal Structure of the alpha-L-arabinofuranosidase UmAbf62A from Ustilago maidys

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-20
Detector	OXFORD ONYX CCD
Wavelength(s)	0.8266
Spacegroup name	P 21 21 21
Unit cell lengths	60.280, 65.900, 68.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.300 - 1.000
R-factor	0.11496
Rwork	0.114
R-free	0.13946
Structure solution method	SAD
RMSD bond length	0.022
RMSD bond angle	2.293
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHELX (CDE)
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	37.300	37.300	1.030
High resolution limit [Å]	1.000	4.480	1.000
Rmerge	0.098	0.064	0.642
Number of reflections	145655
<I/σ(I)>	10	30.16	1.96
Completeness [%]	99.4	99.4	95.4
Redundancy	5.5	8.3	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	285	20% (w/v) PEG 3350, 0.2 M Sodium phosphate, 0.05 M Na Acetate, VAPOR DIFFUSION, HANGING DROP, temperature 285.0K
1	VAPOR DIFFUSION, HANGING DROP	5	285	20% (w/v) PEG 3350, 0.2 M Sodium phosphate, 0.05 M Na Acetate, VAPOR DIFFUSION, HANGING DROP, temperature 285.0K