4N1D

Nodal/BMP2 chimera NB250

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-02-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	H 3 2
Unit cell lengths	95.570, 95.570, 97.494
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.548 - 1.912
R-factor	0.1784
Rwork	0.175
R-free	0.20390
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.136
Data reduction software	XDS
Data scaling software	SCALA (3.3.16)
Phasing software	MrBUMP
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	31.548	31.548	2.020
High resolution limit [Å]	1.912	6.050	1.910
Rmerge		0.015	0.342
Total number of observations		4282	13501
Number of reflections	13316
<I/σ(I)>	41.4	40.9	2.3
Completeness [%]	99.2	99.4	94.3
Redundancy	9.7	9.2	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	288	0.2M NaCl, 0.1 M Na acetate pH 4.6, 30% 2-Methyl-2,4-pentanediol, VAPOR DIFFUSION, HANGING DROP, temperature 288K