4N12
Crystal structure of human E18D DJ-1 in complex with Cu
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-11 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.3702 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 75.110, 75.110, 75.350 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.860 - 1.478 |
R-factor | 0.1644 |
Rwork | 0.164 |
R-free | 0.17420 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.163 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHENIX (MR) |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.860 | 1.560 |
High resolution limit [Å] | 1.478 | 1.478 |
Rmerge | 0.080 | 0.185 |
Number of reflections | 41496 | |
Completeness [%] | 99.9 | 100 |
Redundancy | 11.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.2 M Sodium Malonate and 20% w/v PEG 3350, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |