4N0O
Complex structure of Arterivirus nonstructural protein 10 (helicase) with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 130 |
| Detector technology | CCD |
| Collection date | 2012-03-04 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 56.628, 88.838, 128.781 |
| Unit cell angles | 81.74, 90.01, 71.42 |
Refinement procedure
| Resolution | 50.000 - 2.650 |
| R-factor | 0.24672 |
| Rwork | 0.246 |
| R-free | 0.26353 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4n0n |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.334 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.700 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Number of reflections | 66954 | |
| Completeness [%] | 96.1 | 95.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 1.2M NH4SO4, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
