4N0O
Complex structure of Arterivirus nonstructural protein 10 (helicase) with DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 130 |
Detector technology | CCD |
Collection date | 2012-03-04 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1 |
Spacegroup name | P 1 |
Unit cell lengths | 56.628, 88.838, 128.781 |
Unit cell angles | 81.74, 90.01, 71.42 |
Refinement procedure
Resolution | 50.000 - 2.650 |
R-factor | 0.24672 |
Rwork | 0.246 |
R-free | 0.26353 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4n0n |
RMSD bond length | 0.008 |
RMSD bond angle | 1.334 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.700 |
High resolution limit [Å] | 2.650 | 2.650 |
Number of reflections | 66954 | |
Completeness [%] | 96.1 | 95.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 1.2M NH4SO4, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 277K |