4N0I

Crystal structure of S. cerevisiae mitochondrial GatFAB in complex with glutamine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-12-17
Detector	ADSC QUANTUM 210
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	62.438, 85.426, 194.784
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.458 - 2.001
R-factor	0.1906
Rwork	0.189
R-free	0.21920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Yeast GatFAB free form
RMSD bond length	0.006
RMSD bond angle	1.060
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX (1.7.2_869)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.030
High resolution limit [Å]	2.000	5.430	2.000
Rmerge	0.071	0.050	0.332
Number of reflections	68988
<I/σ(I)>	15.7
Completeness [%]	96.9	90.2	97.5
Redundancy	5.3	6	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	50mM Sodium malonate, 200mM Mg(NO3)2, 10% PEG3350, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K