4N0B
Crystal structure of Bacillus subtilis GabR, an autorepressor and transcriptional activator of GabT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Wavelength(s) | 1.0039 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 97.248, 101.330, 211.215 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.634 - 2.705 |
| R-factor | 0.2181 |
| Rwork | 0.216 |
| R-free | 0.25830 |
| Structure solution method | SAD combined with MR |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.552 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.3_1472) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.710 |
| Number of reflections | 57413 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
