4N08

Structure of Trypanosoma brucei brucei adenosine kinase (apo)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-07-11
Detector	PSI PILATUS 6M
Wavelength(s)	0.97630
Spacegroup name	P 41 21 2
Unit cell lengths	60.084, 60.084, 192.856
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.900 - 2.600
R-factor	0.16999
Rwork	0.167
R-free	0.23082
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.465
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0029)

Data quality characteristics

	Overall
Low resolution limit [Å]	57.360
High resolution limit [Å]	2.600
Number of reflections	11649

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.8	277	0.1 M Tris, pH 8.8, 60% (v/v) MPD, VAPOR DIFFUSION, HANGING DROP, temperature 277K