4N04
The crystal structure of glyoxalase / bleomycin resistance protein from Catenulispora Acidiphila DSM 44928
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-12-18 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9789900 |
Spacegroup name | I 21 3 |
Unit cell lengths | 123.600, 123.600, 123.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.086 - 2.489 |
R-factor | 0.1786 |
Rwork | 0.176 |
R-free | 0.22740 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.198 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MLPHARE |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.520 |
High resolution limit [Å] | 2.480 | 2.480 |
Number of reflections | 11068 | |
<I/σ(I)> | 21.5 | 6.6 |
Completeness [%] | 99.0 | 100 |
Redundancy | 18.7 | 19.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 298 | 0.1 M CHES:NaOH pH 9.5 1.0 M Sodium Citrate, VAPOR DIFFUSION, SITTING DROP, temperature 298K |