4N01
The crystal structure of a periplasmic binding protein from Veillonella parvula dsm 2008
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9789666 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 107.192, 46.330, 108.550 |
| Unit cell angles | 90.00, 119.62, 90.00 |
Refinement procedure
| Resolution | 28.141 - 1.797 |
| R-factor | 0.1728 |
| Rwork | 0.171 |
| R-free | 0.20450 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.982 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 82694 | |
| <I/σ(I)> | 8.1 | 2.19 |
| Completeness [%] | 99.6 | 99 |
| Redundancy | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.6 | 298 | 0.2 M Ammonium Formate pH 6.6, 20% (w/v) PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
