4MZ3
Crystal structure of the voltage-gated sodium channel beta 4 subunit extracellular domain, C131W mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-21 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9749 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.574, 42.521, 89.142 |
| Unit cell angles | 90.00, 91.51, 90.00 |
Refinement procedure
| Resolution | 26.082 - 1.741 |
| R-factor | 0.1769 |
| Rwork | 0.175 |
| R-free | 0.21950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mz2 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.172 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.000 | |
| High resolution limit [Å] | 1.740 | 1.740 |
| Number of reflections | 24173 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 20% PEG formate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
