4MYZ
Structure of a class 2 docking domain complex from modules CurK and CurL of the curacin A polyketide synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-03-07 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.033 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 90.889, 59.532, 52.867 |
| Unit cell angles | 90.00, 124.87, 90.00 |
Refinement procedure
| Resolution | 26.450 - 1.500 |
| R-factor | 0.179 |
| Rwork | 0.178 |
| R-free | 0.20276 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.194 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 35689 | |
| <I/σ(I)> | 2.5 | |
| Completeness [%] | 96.9 | 98.7 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 3.4M Ammonium sulfate and 0.1M Bis-Tris propane pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
