4MYV
Free HSV-2 gD structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97916 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.562, 55.351, 65.335 |
| Unit cell angles | 90.00, 96.35, 90.00 |
Refinement procedure
| Resolution | 36.268 - 1.801 |
| R-factor | 0.1907 |
| Rwork | 0.189 |
| R-free | 0.23020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l2g |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.315 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.085 | 0.297 |
| Number of reflections | 40614 | |
| <I/σ(I)> | 15.89 | 2.714 |
| Completeness [%] | 96.8 | 81.2 |
| Redundancy | 3.9 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 291 | 0.1mol/L Hepes pH 7.2, 5% (V/V) MPD, 10% PEG 10000, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
