4MYT

Crystal structure of elongation factor G (EFG)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	90
Detector technology	IMAGE PLATE
Collection date	2012-11-21
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54178
Spacegroup name	P 21 21 21
Unit cell lengths	74.875, 101.356, 114.385
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.930 - 3.505
R-factor	0.2117
Rwork	0.207
R-free	0.30790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m1k
RMSD bond length	0.009
RMSD bond angle	1.355
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.000	3.620
High resolution limit [Å]	3.500	3.500
Number of reflections	11171
Completeness [%]	97.9	97.6
Redundancy		6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	289	5 mM Tris-HCl (pH 7.6), 10mM MgCl2, 5mM GDP, 20-23% PEG 8000, 0.1M Tris-Cl (PH 7.5-7.6), VAPOR DIFFUSION, HANGING DROP, temperature 289K