4MYR
Crystal structure of a putative CpaE2 pilus assembly protein (CpaE2) from Sinorhizobium meliloti 1021 at 2.72 A resolution (PSI Community Target, Shapiro)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-12-08 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 1 |
Unit cell lengths | 47.013, 48.371, 73.869 |
Unit cell angles | 108.23, 90.03, 97.40 |
Refinement procedure
Resolution | 46.578 - 2.720 |
R-factor | 0.1976 |
Rwork | 0.195 |
R-free | 0.25300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4n0p |
RMSD bond length | 0.010 |
RMSD bond angle | 1.120 |
Data reduction software | XDS |
Data scaling software | XSCALE (December 6, 2010) |
Phasing software | PHASER (2.3.0) |
Refinement software | BUSTER-TNT (2.10.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.578 | 46.578 | 2.820 |
High resolution limit [Å] | 2.720 | 5.820 | 2.720 |
Rmerge | 0.087 | 0.044 | 0.681 |
Number of reflections | 14731 | 1502 | 1516 |
<I/σ(I)> | 10.96 | 24.4 | 2 |
Completeness [%] | 88.1 | 89 | 89.4 |
Redundancy | 3.58 | 3.62 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | 30.00% MPD, 0.1M MES pH 6.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |