4MYP
Structure of the central NEAT domain, N2, of the listerial Hbp2 protein complexed with heme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-06-06 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 32.730, 67.690, 58.480 |
Unit cell angles | 90.00, 91.62, 90.00 |
Refinement procedure
Resolution | 21.050 - 1.800 |
R-factor | 0.196 |
Rwork | 0.195 |
R-free | 0.22500 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.060 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.5.2) |
Refinement software | BUSTER-TNT (BUSTER 2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 21.050 | 1.850 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.046 | 0.423 |
Number of reflections | 22884 | |
<I/σ(I)> | 14.1 | 2.8 |
Completeness [%] | 96.7 | 95.4 |
Redundancy | 3.2 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | 298 | 100 mM MES (pH 6.0), 10 mM ZnCl2, 20% polyethylene glycol 6,000, vapor diffusion, temperature 298K, VAPOR DIFFUSION |