4MYC

Structure of the mitochondrial ABC transporter, Atm1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-08-15
Detector	PSI PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 65 2 2
Unit cell lengths	156.618, 156.618, 520.562
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.600 - 3.060
R-factor	0.253
Rwork	0.251
R-free	0.29790
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.354
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	SHELXCD
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	48.600
High resolution limit [Å]	3.060
Number of reflections	76568
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	283	35% (w/v) PEE (pentaerythritol ethoxylate), 100mM MES, pH 6.5, 200mM ammonium sulphate, VAPOR DIFFUSION, HANGING DROP, temperature 283K