4MY5
Crystal structure of the aromatic amino acid aminotransferase from Streptococcus mutants
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-03-05 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9793 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 104.754, 64.257, 125.600 |
Unit cell angles | 90.00, 106.77, 90.00 |
Refinement procedure
Resolution | 52.493 - 2.194 |
R-factor | 0.1934 |
Rwork | 0.191 |
R-free | 0.23880 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.251 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 56.690 | 56.690 | 2.270 |
High resolution limit [Å] | 2.190 | 4.720 | 2.190 |
Rmerge | 0.069 | ||
Number of reflections | 76921 | ||
Completeness [%] | 93.0 | 97 | 60.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 0.2M potassium chloride pH 7.0, 20%(w/v) polyethylene glycol 3350, VAPOR DIFFUSION, HANGING DROP, temperature 291K |