4MY3
Crystal Structure of GCN5-related N-acetyltransferase from Kribbella flavida
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97937 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 89.246, 167.120, 183.169 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.966 - 2.570 |
| R-factor | 0.179 |
| Rwork | 0.176 |
| R-free | 0.23500 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.184 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1161)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.610 |
| High resolution limit [Å] | 2.570 | 2.570 |
| Number of reflections | 90603 | |
| <I/σ(I)> | 8.7 | 1.8 |
| Completeness [%] | 98.6 | 98.6 |
| Redundancy | 4.4 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.2 M magnesium chloride, 0.1 M MES pH 6.5, 10 %(w/v) PEG400, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
