4MXT
Crystal structure of an Outer-membrane lipoprotein carrier protein (BACUNI_04723) from Bacteroides uniformis ATCC 8492 at 1.40 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.91837,0.97971,0.97894 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 73.262, 42.802, 64.550 |
| Unit cell angles | 90.00, 104.53, 90.00 |
Refinement procedure
| Resolution | 27.049 - 1.400 |
| R-factor | 0.1443 |
| Rwork | 0.143 |
| R-free | 0.17330 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.365 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | PHENIX (1.8.2) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 27.049 | 1.450 | |
| High resolution limit [Å] | 1.400 | 3.010 | 1.400 |
| Rmerge | 0.033 | 0.021 | 0.380 |
| Number of reflections | 36767 | 6655 | 5956 |
| <I/σ(I)> | 10.3 | 25.3 | 1.7 |
| Completeness [%] | 89.8 | 88.3 | 79.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.20M di-ammonium tartrate, 20.)% polyethylene glycol 3350, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
