4MXQ
42F3 TCR pCPC5/H-2Ld Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-02-10 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 102.719, 102.719, 322.944 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.334 - 2.596 |
| R-factor | 0.2121 |
| Rwork | 0.211 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.773 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.750 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 31125 | |
| Completeness [%] | 96.7 | 98.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 14% PEG 3350, 100mM BIS-TRIS, pH 7.0 and 200mM magnesium nitrate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
