4MX8
Crystal Structure of TroA-like Periplasmic Binding Protein Peripla_BP_2 from Xylanimonas cellulosilytica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97897 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 201.663, 116.547, 177.158 |
| Unit cell angles | 90.00, 112.19, 90.00 |
Refinement procedure
| Resolution | 49.370 - 2.911 |
| R-factor | 0.161 |
| Rwork | 0.159 |
| R-free | 0.19600 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.137 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: dev_1367)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.950 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 81145 | |
| <I/σ(I)> | 11.7 | 2.2 |
| Completeness [%] | 98.2 | 96.4 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 2.4 M sodium malonate pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
