4MX6
Crystal structure of a trap periplasmic solute binding protein from shewanella oneidensis (SO_3134), target EFI-510275, with bound succinate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-08-14 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.066, 68.581, 53.937 |
| Unit cell angles | 90.00, 99.66, 90.00 |
Refinement procedure
| Resolution | 26.587 - 1.100 |
| R-factor | 0.1343 |
| Rwork | 0.133 |
| R-free | 0.15270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.184 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | AMoRE |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 68.581 | 68.581 | 1.160 |
| High resolution limit [Å] | 1.100 | 3.480 | 1.100 |
| Rmerge | 0.056 | 0.021 | 0.583 |
| Total number of observations | 11127 | 46390 | |
| Number of reflections | 109476 | ||
| <I/σ(I)> | 11.1 | 15 | 1.4 |
| Completeness [%] | 99.1 | 91.2 | 97.5 |
| Redundancy | 3.5 | 3.4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | Protein (47.1 mg/ml, 10 mM Hepes, 10 mM Succinc Acid), Reservoir (40 % v/v pentaerythritol propoxylate (5/4 PO/OH), 15 % v/v ethanol (MIDAS C6)), Cryoprotection (Straight), pH 7.5, VAPOR DIFFUSION, HANGING DROP |
