4MW3
Structural Basis for Ca2+ Selectivity of a Voltage-gated Calcium Channel
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.75 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 177.755, 178.270, 130.950 |
| Unit cell angles | 90.00, 132.63, 90.00 |
Refinement procedure
| Resolution | 42.317 - 3.300 |
| R-factor | 0.2504 |
| Rwork | 0.249 |
| R-free | 0.26670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.754 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.360 | 3.480 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.089 | 0.606 |
| Number of reflections | 39450 | |
| <I/σ(I)> | 2.7 | |
| Completeness [%] | 88.0 | 92.3 |
| Redundancy | 5.6 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.75 | 298 | CHAPSO:DMPC bicelles, 0.1M Na-Acetate,pH 4.75, 2M Ammonium Sulfate, 28% Glucose, 15mM Calcium Chloride, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
