4MVQ
Structural Basis for Ca2+ Selectivity of a Voltage-gated Calcium Channel
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-01-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.75 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 177.780, 177.725, 130.820 |
| Unit cell angles | 90.00, 132.79, 90.00 |
Refinement procedure
| Resolution | 27.540 - 3.400 |
| R-factor | 0.28129 |
| Rwork | 0.280 |
| R-free | 0.31319 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.771 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.120 | 3.580 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.103 | 0.636 |
| Number of reflections | 39460 | |
| <I/σ(I)> | 2.7 | |
| Completeness [%] | 96.3 | 98.1 |
| Redundancy | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.75 | 298 | CHAPSO:DMPC BICELLES,0.1M Na-Acetate ,pH4.75, 2M Ammonium Sulfate, 28% Glucose, 15mM Calcium Chloride., VAPOR DIFFUSION, HANGING DROP, temperature 298K |
