4MV9
Crystal Structure of Biotin Carboxylase from Haemophilus influenzae in Complex with Bicarbonate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-12-12 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.98 |
Spacegroup name | P 64 |
Unit cell lengths | 85.628, 85.628, 102.738 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.876 - 1.980 |
R-factor | 0.173 |
Rwork | 0.171 |
R-free | 0.20830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dv1 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.952 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.230 | 2.090 |
High resolution limit [Å] | 1.980 | 1.980 |
Number of reflections | 29598 | |
<I/σ(I)> | 16.2 | 2.3 |
Completeness [%] | 99.4 | 99 |
Redundancy | 2.8 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295.15 | 0.2 M KF, 20% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 295.15K |