4MU5
Crystal structure of murine neuroglobin mutant M144W
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-12 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.932 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 87.636, 87.636, 115.217 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.887 - 1.800 |
| R-factor | 0.2011 |
| Rwork | 0.199 |
| R-free | 0.24920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.431 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.890 | 1.864 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 15838 | |
| Completeness [%] | 98.9 | 98.05 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | 0.1M MES, pH6.5, 1.6M AS, 10% dioxane, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
