4MU0
The structure of wt A. thaliana IGPD2 in complex with Mn2+ and 1,2,4-triazole at 1.3 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-11-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9686 |
| Spacegroup name | P 4 3 2 |
| Unit cell lengths | 113.130, 113.130, 113.130 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.590 - 1.300 |
| R-factor | 0.13049 |
| Rwork | 0.130 |
| R-free | 0.14806 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2f1d |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.525 |
| Data reduction software | XDS ((XIA2)) |
| Data scaling software | XSCALE ((XIA2)) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.590 | 1.330 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.053 | 0.762 |
| Number of reflections | 61097 | |
| <I/σ(I)> | 25.2 | 3.5 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 12.3 | 12.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 290 | 30% PEG200, 0.1 M Tris, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
