4MTQ
Ni-bound GloA2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-26 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.95369 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.145, 74.983, 80.362 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.410 - 2.170 |
R-factor | 0.1853 |
Rwork | 0.183 |
R-free | 0.23290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4mtr |
RMSD bond length | 0.010 |
RMSD bond angle | 1.020 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.840 | 2.280 |
High resolution limit [Å] | 2.170 | 2.170 |
Rmerge | 0.076 | 0.688 |
Number of reflections | 13652 | |
<I/σ(I)> | 16.1 | 2.2 |
Completeness [%] | 98.8 | 98.8 |
Redundancy | 6.6 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.3 | 293 | 0.1 M succinic acid, 21% PEG3350, pH 6.3, VAPOR DIFFUSION, temperature 293K |