4MTP
RdRp from Japanesese Encephalitis Virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-13 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 119.470, 174.010, 182.690 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.777 - 3.650 |
| R-factor | 0.2287 |
| Rwork | 0.226 |
| R-free | 0.27260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.682 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.784 | 3.693 |
| High resolution limit [Å] | 3.650 | 3.650 |
| Number of reflections | 81030 | |
| <I/σ(I)> | 2.4 | |
| Completeness [%] | 99.1 | 99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 10-15% PEG 5000 mono methyl ether, 100mM Tris-Cl pH 8.0, 200mM NaSCN, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
