4MTM
Crystal structure of the tail fiber gp53 from Acinetobacter baumannii bacteriophage AP22
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 103 |
Detector technology | CCD |
Collection date | 2011-10-29 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9200 |
Spacegroup name | H 3 2 |
Unit cell lengths | 51.200, 51.200, 302.030 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.719 - 1.368 |
R-factor | 0.1009 |
Rwork | 0.099 |
R-free | 0.13170 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.317 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SHELX (C/D/E) |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.990 | 1.440 |
High resolution limit [Å] | 1.370 | 1.370 |
Rmerge | 0.056 | |
Number of reflections | 62273 | |
<I/σ(I)> | 33.3 | |
Completeness [%] | 96.6 | 99.2 |
Redundancy | 10.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 24% PEG 4000, 0.225M lithium sulphate, 0.1M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |