4MT8
Structure of the ERS1 dimerization and histidine phosphotransfer domain from Arabidopsis thaliana
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-03-05 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.923 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 75.042, 98.747, 77.177 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.300 - 1.900 |
| R-factor | 0.1964 |
| Rwork | 0.195 |
| R-free | 0.22650 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.509 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | PHENIX ((phenix.refine: dev_1092)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.300 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 22944 | |
| <I/σ(I)> | 14.3 | |
| Completeness [%] | 99.4 | 97.6 |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.18 M l-proline, 0.1 M HEPES pH 7.5, 9% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
