4MT4
Crystal structure of the Campylobacter jejuni CmeC outer membrane channel
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 91.827, 146.324, 418.866 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 100.000 - 2.373 |
| R-factor | 0.2114 |
| Rwork | 0.210 |
| R-free | 0.24320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.492 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_1439) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 100.000 | 2.460 |
| High resolution limit [Å] | 2.370 | 4.930 | 2.370 |
| Rmerge | 0.109 | 0.072 | 0.488 |
| Number of reflections | 204082 | ||
| <I/σ(I)> | 5.1 | ||
| Completeness [%] | 93.7 | 93.3 | 86.6 |
| Redundancy | 1.8 | 1.8 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5 | 298 | 30 PEG400, 0.2M NK4SO4, 0.1M NaAC (5.0), VAPOR DIFFUSION, temperature 298K |
