4MST
Crystal Structure of a putative catalytic domain of a chitinase-like protein (HbCLP1) from Hevea brasiliensis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-12-02 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.978570 |
Spacegroup name | I 1 2 1 |
Unit cell lengths | 111.106, 40.415, 111.854 |
Unit cell angles | 90.00, 96.73, 90.00 |
Refinement procedure
Resolution | 41.658 - 1.927 |
R-factor | 0.1676 |
Rwork | 0.166 |
R-free | 0.20210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3cql |
RMSD bond length | 0.011 |
RMSD bond angle | 1.109 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.3_1479)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 83.316 | 41.620 | 2.060 |
High resolution limit [Å] | 1.927 | 6.170 | 1.950 |
Rmerge | 0.088 | 0.041 | 0.519 |
Number of reflections | 37443 | ||
<I/σ(I)> | 14.16 | 30 | 4.4 |
Completeness [%] | 99.3 | 99.8 | 99.5 |
Redundancy | 6.6 | 6.5 | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.3 | 291 | 7 mg/mL protein, 0.1 M Tris-HCl, pH 8.3, 0.2 M magnesium chloride, 30% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 291K |