4MS9

Native RNA-10mer Structure: ccggcgccgg

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-01-10
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	P 31 2 1
Unit cell lengths	22.708, 22.708, 77.721
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.910 - 1.320
R-factor	0.19016
Rwork	0.188
R-free	0.22753
Structure solution method	AB INITIO PHASING
RMSD bond length	0.015
RMSD bond angle	1.706
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	ACORN
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.370
High resolution limit [Å]	1.320	1.320
Rmerge	0.055	0.225
Number of reflections	5742
<I/σ(I)>	33.1	8.28
Completeness [%]	96.0	84.7
Redundancy	11.8	10.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	10% MPD (2-methyl-2,4-pentanediol), 40 mM sodium cacodylate pH 6.0, 12 mM spermine tetrahydrochloride, and 80 mM strontium (II) chloride , VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	6	293	10% MPD (2-methyl-2,4-pentanediol), 40 mM sodium cacodylate pH 6.0, 12 mM spermine tetrahydrochloride, and 80 mM strontium (II) chloride , VAPOR DIFFUSION, HANGING DROP, temperature 293K