4MRS
Structure of a bacterial Atm1-family ABC transporter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-04-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.737 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 322.173, 95.401, 79.398 |
| Unit cell angles | 90.00, 101.81, 90.00 |
Refinement procedure
| Resolution | 39.663 - 2.350 |
| R-factor | 0.1949 |
| Rwork | 0.193 |
| R-free | 0.22270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.245 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | REFMAC ((rigid body)) |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.000 |
| High resolution limit [Å] | 2.345 |
| Number of reflections | 95204 |
| Completeness [%] | 96.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.4 | 277 | 100 mM sodium citrate pH 5.4, 200 mM magnesium acetate, and 12% w/v polyethylene glycol 5,000 mono methyl ether, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
