4MRR
Structure of a bacterial Atm1-family ABC transporter
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9686 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 322.763, 95.947, 81.133 |
Unit cell angles | 90.00, 101.78, 90.00 |
Refinement procedure
Resolution | 39.496 - 2.970 |
R-factor | 0.2045 |
Rwork | 0.203 |
R-free | 0.23620 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.290 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | REFMAC ((rigid body)) |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 40.000 |
High resolution limit [Å] | 2.970 |
Number of reflections | 49540 |
Completeness [%] | 98.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.4 | 277 | 100 mM sodium citrate pH 5.4, 200 mM magnesium acetate, and 12% w/v polyethylene glycol 5,000 mono methyl ether, VAPOR DIFFUSION, SITTING DROP, temperature 277K |