4MR1
X-ray structure of the adduct between hen egg white lysozyme and cis-diamminediiodoplatinum(II)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Collection date | 2013-06-03 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 77.017, 77.017, 36.834 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.230 - 1.990 |
R-factor | 0.184 |
Rwork | 0.184 |
R-free | 0.23400 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.019 |
RMSD bond angle | 4.000 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | CNS (1.3) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.990 |
Rmerge | 0.109 |
Number of reflections | 8113 |
Completeness [%] | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 298 | HEWL crystals were grown at 293 K using the hanging drop vapour diffusion method. The reservoir (600 UL) contained 20 mM sodium acetate buffer at pH 4.2 and 1.0 M NaCl; the drop (0.5+0.5 UL) contained hen egg white lysozyme (10 mg mL-1) in the same acetate buffer (5mM) and reservoir solution. Crystals grow in 1 day. After 5 days, HEWL crystals were soaked in a DMSO solution of cis-PtI2(NH3)2. Final concentration of the metal complex in solution was >10 times higher than HEWL, VAPOR DIFFUSION, HANGING DROP, temperature 298K |