4MQD
Crystal structure of ComJ, inhibitor of the DNA degrading activity of NucA, from Bacillus subtilis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-02-07 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97929 |
Spacegroup name | P 32 |
Unit cell lengths | 125.826, 125.826, 37.013 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.220 - 2.160 |
R-factor | 0.1811 |
Rwork | 0.178 |
R-free | 0.24040 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.845 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.190 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.111 | 0.974 |
Number of reflections | 35026 | |
<I/σ(I)> | 12.3 | 1.54 |
Completeness [%] | 99.7 | 99.9 |
Redundancy | 4.6 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 297 | 0.1M sodium acetate pH 4.5, 20% PEG 3000, VAPOR DIFFUSION, SITTING DROP, temperature 297K |