4MP3
Staphyloferrin B precursor biosynthetic enzyme selenomethionine-labeled SbnB
Experimental procedure
Experimental method | SAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-24 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97649 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 62.887, 62.887, 157.633 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.730 - 2.110 |
Rwork | 0.174 |
R-free | 0.21390 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.428 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.730 | |
High resolution limit [Å] | 2.106 | 2.110 |
Number of reflections | 19092 | |
Completeness [%] | 99.7 | 98.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M Bis-tris, 0.2 M LiSO4, 25% PEG 3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |