4MOZ
Fructose-bisphosphate aldolase from Slackia heliotrinireducens DSM 20476
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-11 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97923 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 135.783, 174.202, 203.023 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.150 |
R-factor | 0.1715 |
Rwork | 0.169 |
R-free | 0.22320 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.955 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.170 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.121 | 0.765 |
Number of reflections | 128506 | |
<I/σ(I)> | 18 | 2.2 |
Completeness [%] | 99.8 | 99.5 |
Redundancy | 5.1 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 90 mM HEPES, 1.26M Sodium Citrate, 10% Glycerol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |