4MOR
Pyranose 2-oxidase H450G/V546C double mutant with 3-fluorinated galactose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-02-12 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.946495 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 100.242, 102.469, 137.840 |
Unit cell angles | 90.00, 91.09, 90.00 |
Refinement procedure
Resolution | 49.330 - 1.500 |
R-factor | 0.156 |
Rwork | 0.156 |
R-free | 0.19800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.027 |
RMSD bond angle | 2.283 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.330 | 1.600 |
High resolution limit [Å] | 1.500 | 1.500 |
Number of reflections | 442699 | |
<I/σ(I)> | 11.9 | 1.6 |
Completeness [%] | 99.7 | 99.7 |
Redundancy | 7.4 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 298 | 0.1 M MES, 50 mM MgCl2, 10% (w/v) monomethylether PEG 2000, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |