4MOQ
Pyranose 2-oxidase V546C mutant with 2-fluorinated galactose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-02-17 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 101.724, 101.724, 251.284 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.320 - 1.600 |
R-factor | 0.18181 |
Rwork | 0.182 |
R-free | 0.21180 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.023 |
RMSD bond angle | 2.152 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.320 | 1.700 |
High resolution limit [Å] | 1.600 | 1.600 |
Number of reflections | 173572 | |
<I/σ(I)> | 14.1 | 2.2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 25.3 | 25.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 298 | 0.1 M MES, 50 mM MgCl2, 10% (w/v) monomethylether PEG 2000, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |