4MOL
Pyranose 2-oxidase H167A mutant with 2-fluorinated galactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9191 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 100.291, 102.476, 137.423 |
| Unit cell angles | 90.00, 91.02, 90.00 |
Refinement procedure
| Resolution | 48.010 - 2.000 |
| R-factor | 0.18637 |
| Rwork | 0.186 |
| R-free | 0.22166 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.921 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.010 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 184127 | |
| <I/σ(I)> | 11.5 | 2.4 |
| Completeness [%] | 98.2 | 97.2 |
| Redundancy | 6.9 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 298 | 0.1 M MES, 50 mM MgCl2, 10% (w/v) monomethylether PEG 2000, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
