4MOF
Pyranose 2-oxidase H450G mutant with 2-fluorinated glucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-03 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9600 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 102.340, 102.340, 128.510 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.850 |
| R-factor | 0.15929 |
| Rwork | 0.158 |
| R-free | 0.19353 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.977 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.420 | 1.900 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 58446 | |
| <I/σ(I)> | 17.4 | 3.2 |
| Completeness [%] | 99.4 | 93.8 |
| Redundancy | 14.3 | 11.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 298 | 0.1M MES, 50mM MgCl2, 10% (w/v) monomethylether PEG 2000 , pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
