4MO9
Crystal Structure of TroA-like Periplasmic Binding Protein FepB from Veillonella parvula
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97987 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.402, 75.999, 125.187 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.361 - 1.925 |
| R-factor | 0.168 |
| Rwork | 0.166 |
| R-free | 0.19900 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.035 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: dev_1367)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.960 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Number of reflections | 29285 | |
| <I/σ(I)> | 9.9 | 2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.8 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.2 M Trimethylamine N-oxide, 0.1 M Tris pH 8.5, 20 %(w/v) PEGMME2000, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
